CRISPR, crispr genome editing system - Part 11

Justin Smith, PhD student in genetics about CRISPR

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I work on CRISPR.  I had previously worked with the older technologies mentioned in the podcast (which I thought was a pretty good description of CRISPR to non-scientists). Honestly the potential benefits of CRISPR far out weight the dangers, in my opinion.  CRISPR should allow for better disease research, better stem cell therapies, better genetically modified crops, and better understanding of basic genetics, better metabolic engineering for new fuels and materials, etc.  It is a truly revolutionary technology. However, I don’t think it will be possible to engineer warriors or make elite designer babies with CRISPR alone.  First off we do not know nearly enough about how the genome works to do something like engineer warriors.  Even decades from now...
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CRISPR, the disruptor

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Illustration by Sébastien Thibault Three years ago, Bruce Conklin came across a method that made him change the course of his lab. Conklin, a geneticist at the Gladstone Institutes in San Francisco, California, had been trying to work out how variations in DNA affect various human diseases, but his tools were cumbersome. When he worked with cells from patients, it was hard to know which sequences were important for disease and which were just background noise. And engineering a mutation into cells was expensive and laborious work. “It was a student’s entire thesis to change one gene,” he says. Then, in 2012, he read about a newly published technique1 calledCRISPR that would allow researchers to quickly change the DNA of...
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CRISPR U.S. scientist “thrilled” to receive Spain’s Asturias prize

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U.S. biochemist Jennifer Doudna said Thursday that she was “thrilled” to receive the 2015 Princess of Asturias Award for Technical and Scientific Research for developing a technology that allows genes to be edited and nucleic acids to be manipulated with great precision. “This award emphasizes the importance of science in society and for helping improve the human condition. I hope that (this award) inspires the work of the coming generation of scientists,” she told Efe. Doudna and French biochemist Emmanuelle Charpentier, who shared the prize, are pioneers in applying a technology known as CRISPR-Cas9 that acts like a molecular pair of scissors to cut and replace DNA components with great precision. The 19-member prize jury credited Charpentier and Doudna with...
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CRISPR developers Emmanuelle Charpentier and Jennifer Doudna, Princess of Asturias Award

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Emmanuelle Charpentier and Jennifer Doudna Source: FPA USPA NEWS – The biochemists Emmanuelle Charpentier (France) and Jennifer Doudna (USA) have been bestowed with the 2015 Princess of Asturias Award for Technical and Scientific Research, as made public in Oviedo by the Jury responsible for conferring said Award. This candidature was put forward by Jerónimo López Martínez, President of the Scientific Committee on Antarctic Research (SCAR), 2002 Prince of Asturias Award for International Cooperation. The research carried out by Emmanuelle Charpentier and Jennifer Doudna has meant a revolution in biotechnology. They have developed a genome-editing technology that enables the genome to be rewritten and defective genes to be corrected very economically with an unprecedented level of precision. Inspired by the antiviral...
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US science academies take on human-genome editing

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IHM/National Library of Medicine The 1975 Asilomar conference helped set rules for research on DNA.                   The US National Academy of Sciences (NAS) and the National Academy of Medicine (NAM) will launch a major initiative to develop guidelines for editing human genomes, they said on 18 May. The initiative follows the April announcement that researchers had used a gene-editing system called CRISPR-Cas9 to remove a mutated gene from a human embryo. The work involved a small number of embryos and was only partially successful, but it has sparked wide debate about the ethics of editing human genomes in ways that can be passed on to future generations. Although researchers and ethicists disagree...
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CRISPR Mouse Service in Creative Animodel Accomplishes Conditional Knock-in

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Researchers from the Creative Animodel Institute use CRISPR/Cas genetic regulatory system, manipulating mouse genome with one step into the reporter gene and the conditional allele. Now we just need several weeks rather than a few years time to generate such a complex engineering allele animals and also to construct models of disease or to research gene function using these animals. Creative Animodel Institute said: “We have previously used CRISPR/Cas to mutate gene, but it can not predict the characteristics of these targeted mutations. Now we can define the specificity deletion by two times cutting. We can generate conditional mice one step, easily and very effectively insert DNA fragments of up to 3000 base pairs. In the past, however, we need...
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Genome Editing Spy Style: Self-destructing DNA with CRISPR

Have you ever watched a spy move and felt yourself craving spy gadgets, such as explosive chewing gum, or a tricked out Aston Martin? Well if you are a genome-engineering scientist you can now get your very own spy gadget – self-destructing DNA! But this cool creation isn’t just to fulfill your 007 dreams, it solves two very real issues: environmental spillover of foreign genetic material and protection of intellectual property. Minimizing spillover is important to help prevent unintended effects of targeted genome editors on surrounding cells. Sadly, traditional DNA removal techniques are quite tedious, time consuming, and expensive to construct. Brian Caliando and Christopher Voigt of MIT decided to tackle this issue by employing the famed CRISPR system to...
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Crispr: is it a good idea to ‘upgrade’ our DNA?

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Last year Tony Perry made mice that would have been brown-furred grow up white instead. That Perry, a molecular embryologist at the University of Bath, tweaked their coat colour isn’t new – scientists have been making so-called knock-out mice, in which certain genes are disabled, since the technique was invented in 1989. It is a long and cumbersome procedure that involves combining pieces of DNA in embryonic stem cells and mouse breeding. But Perry, who published his study in December, didn’t use this method. Instead he used a new genome-editing technology that has been taking the scientific world by storm since it was first developed from the bacterial immune system in 2012, and shown to work in human cells in 2013....
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CRISPR Gets Lit Up and Tuned

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By now, most within the biomedical fields have likely at least heard mention of the gene-editing tool known as CRISPR/Cas9. Since 2013, this technique has created a veritable revolution within the disciplines of molecular biology and genetics and is already showing tremendous promise for treating such diseases a cystic fibrosis and sickle cycle, as well as being able to generate new animal models that better mimic human disease. The power of the CRISPR/Cas9 system lies not only in its powerful editing capabilities, but also its speed, simplicity, and compatibility for use within most organisms. However, there is one main drawback for which scientists at the University of Pittsburgh and University of North Carolina believe they may have just found a...
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Using CRISPR, biologists find a way to comprehensively identify anti-cancer drug targets

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Cold Spring Harbor, NY – Imagine having a complete catalog of the best drug targets to hit in a particularly deadly form of cancer. Imagine having a master catalog of such targets for all the major cancer types and subtypes. Scientists at Cold Spring Harbor Laboratory (CSHL) today publish in Nature Biotechnology a method of compiling just such a catalog, using the revolutionary gene-editing technology called CRISPR. CRISPR enables biologists to manipulate the genetic material of cells with unprecedented precision and ease – letter by DNA letter. CSHL Assistant Professor Chris Vakoc, M.D., Ph.D., and Junwei Shi, a Ph.D. student investigator in his lab, have figured out how to harness CRISPR’s elegant power to the task that preoccupies their lab...
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