CRISPR, crispr genome editing system - Part 2

CRISPR: A Billion-Dollar Pie

CrisprMoney1
CRISPR sits in the middle of a costly patent brawl. That hasn’t stopped scientists and venture capitalists from betting on who will win. Business is moving fast with licenses being sold, awaiting the USPTO’s decision in November which may declare a winner on the patent. When it comes to pharmaceuticals, patents are licensed and traded in the amount of millions of dollars funneled through a 14 to 20-year timer. While the stakes are high, CRISPR/Cas9 is not the only gene editing technology out there. It was however, the first gene editing technology to be discovered in ancient bacteria, channeling new interest with the hope to discover an unclaimed and more effective gene editing technique. Who’s Who in the CRISPR Patent Brawl...
Read More..

Scientists shocked by major CRISPR breakthrough

crispr image2
Genetic engineering has popped up in the headlines again after a breakthrough study pushed the CRISPR-Cas9 editing technique to a new level of precision. According to a report from Nature, the technique used for cutting and pasting genes has now been adapted to work for cutting RNA. The breakthrough could give researchers an entire new set of tools for studying and attempting to treat diseases. The study was published by a team led by Feng Zhang from the Broad Institute. Researchers used CRISPR, which stands for “clustered regularly interspaced short palindromic repeats,” to begin splicing and rearranging RNA strands. Published in the journal Science, the findings could lead to techniques that could significantly reduce the burden of a number of...
Read More..

USDA announces it will stop regulating all GMO crops altered with CRISPR gene editing technique

14576502845065
As reported by Business Insider, the U.S. Department of Agriculture released a letter to the public last week confirming that the agency does not plan to regulate a mushroom thathas been genetically modified so that it won’t turn brown. The letter was in response to an inquiry from mushroom developer Dr. Yinong Yang, of the College of Agricultural Services at Penn State University, about whether a division of the USDA would be seeking to regulate the mushroom. This decision, wherever it ultimately came from, is in contrast to the USDA’s previous approach with GMOs, which are regulated by the department’s Animal and Plant Health Inspection Service (APHIS). That division supposedly monitors new genetically modified foods that “may pose a risk...
Read More..

CRISPR Treatment for HIV reportedly three years away

crisprit
An end to HIV and Supports reportedly could be simply a couple of years away after researchers have had the opportunity to successfully snip away the disease from infected skin cells and prevent the condition from returning.  Researchers at the Lewis Katz College of Medication at Temple University or college are confident that next three years they’ll be able to start out human trials, the united kingdom Daily Telegraph reported. American researchers have previously shown that it’s possible to edit genes to slice the virus from DNA cells entirely. British experts assume that this treatment, which includes only been tried in labs, allows your body to “cure itself from the within effectively,” the newspaper reported. People immune cells which were...
Read More..

CRISPR could eventually allow us to treat HIV, cancer, autism

White blood cells
Researchers have managed to devise a new method using gene-editing tool CRISPR-Cas9 that can track movement of RNA in living cells thereby enabling them to study a wide range of disease-related RNA processes. The study, published in Cell, paves way for even greater promise of finding a cure for deadly diseases like HIV and cancer. The work, according to researchers at University of California, San Diego, is just the tip of the iceberg and holds immense potential as it opens a whole new door for treating diseases by correcting disease-causing RNA behaviors – something that wasn’t possible until now considering that CRISPR-Cas9 only targeted DNA. Researchers acknowledge that there are methods that enable them to recognize and track RNA, but...
Read More..

Two New Techniques May Enhance Genetic Engineering With CRISPR

crispr_illo
Scientists have discovered two new methods capable of enhancing the efficiency of genetic engineering, and these new gene modification techniques could be huge when it comes to future research studies. The two new methods are called the IsODN (long single-stranded oligodeoxynucleotide) and 2H2OP (two-hit two-oligo with plasmid) and are used in conjunction with CRISPR-Cas systems and ssODN (single-stranded oligodeoxynucleotide). CRISPR-Cas systems, in particular, have greatly helped with gene modification in mice and rats. This system involves using Cas9 messenger RNA and gRNA. The gRNA recognizes targeted DNA, and then Cas9 cuts the targeting site. This, effectively, leaves a hole that is then repaired by non-homologous end joining; this causes DNA mutations, resulting in gene knock-out. Similar to this system is...
Read More..

CRISPR Critters: Regulators and the New Gene Revolution in Agriculture

14576502845065
It’s CRISPR’s world, and we’re just living in it. The gene-editing technology, which has been likened to a genetic word processor, allows researchers to make specific changes ranging from tweaking a single DNA base pair to revising whole paragraphs of genetic information. Most of CRISPR’s media attention has focused on the vast improvements it could bring to medicine, such as fixing genetic diseases, curing cancer, speeding up vaccine development, andovercoming antibiotic resistance in disease-causing microbes. Less widely reported, but no less momentous, are the dramatic transformations that CRISPR will bring to nearly all aspects of farming. All that is needed for this new green revolution to take off is for regulators to get out of the way. Thanks to entirely...
Read More..

Improved CRISPR System Reads Induced Pluripotent Stem Cell Genome For the First Time

crispr_spiral
With an enhanced form of the CRISPR-Cas9 system, scientists were able to read the genome of induced pluripotent stem cells (iPSCs) for the first time. The new system provides a more temporary gene modification that will be extremely useful for scientists using this method to conduct therapeutic gene editing techniques. The new study builds off of a 2013 method developed by Stanly Qi, PhD, who is also a co-author on the current paper. Qi designed CRISPR interference (CRISPRi), a system with the ability to “inactivate genes in IPSCs and heart cells created from iPSCs.” CRISPRi improves the original system because genes can be silenced or turned off “more precisely and efficiently.” Plus, CRISPRi offers additional beneficial characteristics like deletion flexibility...
Read More..

CRISPR Design Tools for Genome Editing: the full list with description

crispr tools target
In just four years, CRISPR has revolutionized approaches to genome editing. Inexpensive to build and easy to use, CRISPR (Clustered, Regularly Interspaced. Short Palindromic Repeats) has gained worldwide attention for its ability to target specific locations in the genetic code of any organism with just two elements – guide RNA and an enzyme called Cas9. CRISPR was originally discovered as a genetic phenomenon in archaea and certain bacteria – an immune response that could be adapted for purposes of modifying the genes of higher organisms. Applications of crispr system design include genetic moderation of crops, gene therapies for diseases such as cancer, and synthesizing drug-producing microbes. CRISPR’s applications may be even more far-reaching in the future – but some fine-tuning...
Read More..